Combination of dextran sulfate and recombinant trypsin on aggregation of Chinese hamster ovary cells

In laboratory scale therapeutical protein production, cell clumps form typically in shake flasks, which hinders cell growth and decreases protein yield. To minimize clumps during the culture of Chinese hamster ovary cells, we employed the combination of two reagents, dextran sulfate (DS) and recombi...

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Enregistré dans:
Détails bibliographiques
Publié dans:Cytotechnology
Auteurs principaux: Jing, Yu, Zhang, Cunchao, Fu, Tuo, Jiang, Cheng, Ma, Kai, Zhang, Dapeng, Hou, Sheng, Dai, Jianxin, Wang, Hao, Zhang, Xueguang, Kou, Geng, Guo, Yajun
Format: Artigo
Langue:Inglês
Publié: Springer Netherlands 2014
Sujets:
Method in Cell Science
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4754253/
https://ncbi.nlm.nih.gov/pubmed/25087075
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-014-9774-4
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