Combination of dextran sulfate and recombinant trypsin on aggregation of Chinese hamster ovary cells

In laboratory scale therapeutical protein production, cell clumps form typically in shake flasks, which hinders cell growth and decreases protein yield. To minimize clumps during the culture of Chinese hamster ovary cells, we employed the combination of two reagents, dextran sulfate (DS) and recombi...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Cytotechnology
Hauptverfasser: Jing, Yu, Zhang, Cunchao, Fu, Tuo, Jiang, Cheng, Ma, Kai, Zhang, Dapeng, Hou, Sheng, Dai, Jianxin, Wang, Hao, Zhang, Xueguang, Kou, Geng, Guo, Yajun
Format: Artigo
Sprache:Inglês
Veröffentlicht: Springer Netherlands 2014
Schlagworte:
Method in Cell Science
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4754253/
https://ncbi.nlm.nih.gov/pubmed/25087075
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-014-9774-4
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