Combination of dextran sulfate and recombinant trypsin on aggregation of Chinese hamster ovary cells

In laboratory scale therapeutical protein production, cell clumps form typically in shake flasks, which hinders cell growth and decreases protein yield. To minimize clumps during the culture of Chinese hamster ovary cells, we employed the combination of two reagents, dextran sulfate (DS) and recombi...

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Bibliografiske detaljer
Udgivet i:Cytotechnology
Main Authors: Jing, Yu, Zhang, Cunchao, Fu, Tuo, Jiang, Cheng, Ma, Kai, Zhang, Dapeng, Hou, Sheng, Dai, Jianxin, Wang, Hao, Zhang, Xueguang, Kou, Geng, Guo, Yajun
Format: Artigo
Sprog:Inglês
Udgivet: Springer Netherlands 2014
Fag:
Method in Cell Science
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4754253/
https://ncbi.nlm.nih.gov/pubmed/25087075
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-014-9774-4
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