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Effect of Zn(2+) binding and enzyme active site on the transition state for RNA 2′-O-transphosphorylation interpreted through kinetic isotope effects

Divalent metal ions, due to their ability to stabilize high concentrations of negative charge, are important for RNA folding and catalysis. Detailed models derived from the structures and kinetics of enzymes and from computational simulations have been developed. However, in most cases the specific...

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Publicado en:Biochim Biophys Acta
Autores principales: Chen, Haoyuan, Piccirilli, Joseph A., Harris, Michael E., York, Darrin M.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2015
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4743498/
https://ncbi.nlm.nih.gov/pubmed/25812974
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbapap.2015.02.022
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