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Immunochemical detection of 4-hydroxynonenal protein adducts in oxidized hepatocytes.

We report here the development of an immunochemical procedure that uses an antibody specific to the 4-hydroxynonenal (HNE) moiety for the detection of HNE-protein adducts. The HNE-specific antibody was prepared by immunizing rabbits with a HNE-keyhole limpet hemocyanin conjugate and purifying the ra...

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Autors principals: Uchida, K, Szweda, L I, Chae, H Z, Stadtman, E R
Format: Artigo
Idioma:Inglês
Publicat: 1993
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC47434/
https://ncbi.nlm.nih.gov/pubmed/8378358
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