Immunochemical detection of 4-hydroxynonenal protein adducts in oxidized hepatocytes.

We report here the development of an immunochemical procedure that uses an antibody specific to the 4-hydroxynonenal (HNE) moiety for the detection of HNE-protein adducts. The HNE-specific antibody was prepared by immunizing rabbits with a HNE-keyhole limpet hemocyanin conjugate and purifying the ra...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Uchida, K, Szweda, L I, Chae, H Z, Stadtman, E R
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1993
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC47434/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8378358/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.18.8742
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