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Sequence features associated with the cleavage efficiency of CRISPR/Cas9 system

The CRISPR-Cas9 system has recently emerged as a versatile tool for biological and medical research. In this system, a single guide RNA (sgRNA) directs the endonuclease Cas9 to a targeted DNA sequence for site-specific manipulation. In addition to this targeting function, the sgRNA has also been sho...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Liu, Xiaoxi, Homma, Ayaka, Sayadi, Jamasb, Yang, Shu, Ohashi, Jun, Takumi, Toru
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4728555/
https://ncbi.nlm.nih.gov/pubmed/26813419
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep19675
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