PINCER: improved CRISPR/Cas9 screening by efficient cleavage at conserved residues

CRISPR/Cas9 functional genomic screens have emerged as essential tools in drug target discovery. However, the sensitivity of available genome-wide CRISPR libraries is impaired by guides which inefficiently abrogate gene function. While Cas9 cleavage efficiency optimization and essential domain targe...

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Bibliographische Detailangaben
Veröffentlicht in:Nucleic Acids Res
Hauptverfasser: Veeneman, Brendan, Gao, Ying, Grant, Joy, Fruhling, David, Ahn, James, Bosbach, Benedikt, Bienkowska, Jadwiga, Follettie, Maximillian, Arndt, Kim, Myers, Jeremy, Zhong, Wenyan
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2020
Schlagworte:
Computational Biology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7515706/
https://ncbi.nlm.nih.gov/pubmed/32821942
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa645
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