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Replacement of Y(730) and Y(731) in the α2 Subunit of Escherichia coli Ribonucleotide Reductase with 3-Aminotyrosine using an Evolved Suppressor tRNA/tRNA-Synthetase Pair
Since the discovery of the essential tyrosyl radical (Y•) in E. coli ribonucleotide reductase (RNR), a number of enzymes involved in primary metabolism have been found that use transient or stable tyrosyl (Y) or tryptophanyl (W) radicals in catalysis. These enzymes engage in a myriad of charge trans...
Gardado en:
| Publicado en: | Methods Enzymol |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2009
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4712453/ https://ncbi.nlm.nih.gov/pubmed/19632469 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S0076-6879(09)62003-6 |
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