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An Evolved Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase/tRNA Pair Is Highly Active and Orthogonal in Mammalian Cells

[Image: see text] We recently characterized a new class of pyrrolysyl-tRNA synthetase (PylRS)/(Pyl)tRNA pairs from Methanomassiliicocales that are active and orthogonal in Escherichia coli. The aminoacyl-tRNA synthetases (aaRSs) of these pairs lack the N-terminal domain that is essential for tRNA re...

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Bibliografiske detaljer
Udgivet i:Biochemistry
Main Authors: Beránek, Václav, Willis, Julian C. W., Chin, Jason W.
Format: Artigo
Sprog:Inglês
Udgivet: American Chemical Society 2018
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6365905/
https://ncbi.nlm.nih.gov/pubmed/30260626
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.8b00808
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