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An Evolved Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase/tRNA Pair Is Highly Active and Orthogonal in Mammalian Cells
[Image: see text] We recently characterized a new class of pyrrolysyl-tRNA synthetase (PylRS)/(Pyl)tRNA pairs from Methanomassiliicocales that are active and orthogonal in Escherichia coli. The aminoacyl-tRNA synthetases (aaRSs) of these pairs lack the N-terminal domain that is essential for tRNA re...
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| 發表在: | Biochemistry |
|---|---|
| Main Authors: | , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
American
Chemical Society
2018
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| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6365905/ https://ncbi.nlm.nih.gov/pubmed/30260626 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.8b00808 |
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