Formation of reactive oxygen species by human and bacterial pyruvate and 2-oxoglutarate dehydrogenase multienzyme complexes reconstituted from recombinant components

Individual recombinant components of pyruvate and 2-oxoglutarate dehydrogenase multienzyme complexes (PDHc, OGDHc) of human and Escherichia coli (E. coli) origin were expressed and purified from E. coli with optimized protocols. The four multienzyme complexes were each reconstituted under optimal co...

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Publicado en:Free Radic Biol Med
Autores principales: Ambrus, Attila, Nemeria, Natalia S., Torocsik, Beata, Tretter, Laszlo, Nilsson, Mattias, Jordan, Frank, Adam-Vizi, Vera
Formato: Artigo
Lenguaje:Inglês
Publicado: 2015
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4684775/
https://ncbi.nlm.nih.gov/pubmed/26456061
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.freeradbiomed.2015.10.001
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