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Formation of reactive oxygen species by human and bacterial pyruvate and 2-oxoglutarate dehydrogenase multienzyme complexes reconstituted from recombinant components

Individual recombinant components of pyruvate and 2-oxoglutarate dehydrogenase multienzyme complexes (PDHc, OGDHc) of human and Escherichia coli (E. coli) origin were expressed and purified from E. coli with optimized protocols. The four multienzyme complexes were each reconstituted under optimal co...

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發表在:Free Radic Biol Med
Main Authors: Ambrus, Attila, Nemeria, Natalia S., Torocsik, Beata, Tretter, Laszlo, Nilsson, Mattias, Jordan, Frank, Adam-Vizi, Vera
格式: Artigo
語言:Inglês
出版: 2015
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4684775/
https://ncbi.nlm.nih.gov/pubmed/26456061
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.freeradbiomed.2015.10.001
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