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Perturbation of the conformational dynamics of an active-site loop alters enzyme activity

The role of internal dynamics in enzyme function is highly debated. Specifically, how small changes in structure far away from the reaction site alter protein dynamics and overall enzyme mechanisms is of wide interest in protein engineering. Using RNase A as a model, we demonstrate that elimination...

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Bibliografske podrobnosti
izdano v:Structure
Main Authors: Gagné, Donald, French, Rachel L., Narayanan, Chitra, Simonović, Miljan, Agarwal, Pratul K., Doucet, Nicolas
Format: Artigo
Jezik:Inglês
Izdano: 2015
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4680847/
https://ncbi.nlm.nih.gov/pubmed/26655472
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.str.2015.10.011
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