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Perturbation of the conformational dynamics of an active-site loop alters enzyme activity

The role of internal dynamics in enzyme function is highly debated. Specifically, how small changes in structure far away from the reaction site alter protein dynamics and overall enzyme mechanisms is of wide interest in protein engineering. Using RNase A as a model, we demonstrate that elimination...

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Detalhes bibliográficos
Publicado no:Structure
Main Authors: Gagné, Donald, French, Rachel L., Narayanan, Chitra, Simonović, Miljan, Agarwal, Pratul K., Doucet, Nicolas
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4680847/
https://ncbi.nlm.nih.gov/pubmed/26655472
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.str.2015.10.011
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