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Integrated mass spectrometry–based analysis of plasma glycoproteins and their glycan modifications

We present a protocol for the identification of glycosylated proteins in plasma followed by elucidation of their individual glycan compositions. The study of glycoproteins by mass spectrometry is usually based on cleavage of glycans followed by separate analysis of glycans and deglycosylated protein...

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Detalles Bibliográficos
Publicado en:Nat Protoc
Main Authors: Wang, Hong, Wong, Chee-Hong, Chin, Alice, Taguchi, Ayumu, Taylor, Allen, Hanash, Samir, Sekiya, Sadanori, Takahashi, Hidenori, Murase, Masaki, Kajihara, Shigeki, Iwamoto, Shinichi, Tanaka, Koichi
Formato: Artigo
Idioma:Inglês
Publicado: 2011
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4677799/
https://ncbi.nlm.nih.gov/pubmed/21372808
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2010.176
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