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Integrated mass spectrometry–based analysis of plasma glycoproteins and their glycan modifications

We present a protocol for the identification of glycosylated proteins in plasma followed by elucidation of their individual glycan compositions. The study of glycoproteins by mass spectrometry is usually based on cleavage of glycans followed by separate analysis of glycans and deglycosylated protein...

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Detalhes bibliográficos
Publicado no:Nat Protoc
Main Authors: Wang, Hong, Wong, Chee-Hong, Chin, Alice, Taguchi, Ayumu, Taylor, Allen, Hanash, Samir, Sekiya, Sadanori, Takahashi, Hidenori, Murase, Masaki, Kajihara, Shigeki, Iwamoto, Shinichi, Tanaka, Koichi
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4677799/
https://ncbi.nlm.nih.gov/pubmed/21372808
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2010.176
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