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Integrated mass spectrometry–based analysis of plasma glycoproteins and their glycan modifications

We present a protocol for the identification of glycosylated proteins in plasma followed by elucidation of their individual glycan compositions. The study of glycoproteins by mass spectrometry is usually based on cleavage of glycans followed by separate analysis of glycans and deglycosylated protein...

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Détails bibliographiques
Publié dans:Nat Protoc
Auteurs principaux: Wang, Hong, Wong, Chee-Hong, Chin, Alice, Taguchi, Ayumu, Taylor, Allen, Hanash, Samir, Sekiya, Sadanori, Takahashi, Hidenori, Murase, Masaki, Kajihara, Shigeki, Iwamoto, Shinichi, Tanaka, Koichi
Format: Artigo
Langue:Inglês
Publié: 2011
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4677799/
https://ncbi.nlm.nih.gov/pubmed/21372808
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2010.176
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