Integrated mass spectrometry–based analysis of plasma glycoproteins and their glycan modifications

We present a protocol for the identification of glycosylated proteins in plasma followed by elucidation of their individual glycan compositions. The study of glycoproteins by mass spectrometry is usually based on cleavage of glycans followed by separate analysis of glycans and deglycosylated protein...

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Vydáno v:Nat Protoc
Hlavní autoři: Wang, Hong, Wong, Chee-Hong, Chin, Alice, Taguchi, Ayumu, Taylor, Allen, Hanash, Samir, Sekiya, Sadanori, Takahashi, Hidenori, Murase, Masaki, Kajihara, Shigeki, Iwamoto, Shinichi, Tanaka, Koichi
Médium: Artigo
Jazyk:Inglês
Vydáno: 2011
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4677799/
https://ncbi.nlm.nih.gov/pubmed/21372808
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2010.176
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