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Force dependency of biochemical reactions measured by single molecule force-clamp spectroscopy
Here we describe a protocol for using force-clamp spectroscopy to precisely quantify the effect of force on biochemical reactions. A calibrated force is used to control the exposure of reactive sites in a single polyprotein substrate composed of repeated domains. The use of polyproteins allows the i...
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| Publié dans: | Nat Protoc |
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| Auteurs principaux: | , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2013
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4676941/ https://ncbi.nlm.nih.gov/pubmed/23744288 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.056 |
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