Carregant...

Force dependency of biochemical reactions measured by single molecule force-clamp spectroscopy

Here we describe a protocol for using force-clamp spectroscopy to precisely quantify the effect of force on biochemical reactions. A calibrated force is used to control the exposure of reactive sites in a single polyprotein substrate composed of repeated domains. The use of polyproteins allows the i...

Descripció completa

Guardat en:
Dades bibliogràfiques
Publicat a:Nat Protoc
Autors principals: Popa, Ionel, Kosuri, Pallav, Alegre-Cebollada, Jorge, Garcia-Manyes, Sergi, Fernandez, Julio M.
Format: Artigo
Idioma:Inglês
Publicat: 2013
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4676941/
https://ncbi.nlm.nih.gov/pubmed/23744288
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.056
Etiquetes: Afegir etiqueta
Sense etiquetes, Sigues el primer a etiquetar aquest registre!