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Identification of DNA lesions using a third base pair for amplification and nanopore sequencing

Damage to the genome is implicated in the progression of cancer and stress-induced diseases. DNA lesions exist in low levels, and cannot be amplified by standard PCR because they are frequently strong blocks to polymerases. Here, we describe a method for PCR amplification of lesion-containing DNA in...

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Detalhes bibliográficos
Publicado no:Nat Commun
Main Authors: Riedl, Jan, Ding, Yun, Fleming, Aaron M., Burrows, Cynthia J.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Pub. Group 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4667634/
https://ncbi.nlm.nih.gov/pubmed/26542210
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms9807
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