Efficient and quantitative high-throughput transfer RNA sequencing

Despite its biological importance, transfer RNA (tRNA) could not be adequately sequenced by standard methods due to abundant post-transcriptional modifications and stable structure, which interfere with cDNA synthesis. We achieve efficient and quantitative tRNA sequencing using engineered demethylas...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Nat Methods
Hauptverfasser: Zheng, Guanqun, Qin, Yidan, Clark, Wesley C., Dai, Qing, Yi, Chengqi, He, Chuan, Lambowitz, Alan M., Pan, Tao
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2015
Schlagworte:
Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4624326/
https://ncbi.nlm.nih.gov/pubmed/26214130
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3478
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!

Ähnliche Einträge