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Efficient and quantitative high-throughput transfer RNA sequencing
Despite its biological importance, transfer RNA (tRNA) could not be adequately sequenced by standard methods due to abundant post-transcriptional modifications and stable structure, which interfere with cDNA synthesis. We achieve efficient and quantitative tRNA sequencing using engineered demethylas...
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Publicado no: | Nat Methods |
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Main Authors: | , , , , , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2015
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4624326/ https://ncbi.nlm.nih.gov/pubmed/26214130 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3478 |
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