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Balancing between affinity and speed in target DNA search by zinc-finger proteins via modulation of dynamic conformational ensemble

Although engineering of transcription factors and DNA-modifying enzymes has drawn substantial attention for artificial gene regulation and genome editing, most efforts focus on affinity and specificity of the DNA-binding proteins, typically overlooking the kinetic properties of these proteins. Howev...

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Pubblicato in:Proc Natl Acad Sci U S A
Autori principali: Zandarashvili, Levani, Esadze, Alexandre, Vuzman, Dana, Kemme, Catherine A., Levy, Yaakov, Iwahara, Junji
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 2015
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4577168/
https://ncbi.nlm.nih.gov/pubmed/26324943
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1507726112
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