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Thermodynamic additivity for impacts of base-pair substitutions on association of the Egr-1 zinc-finger protein with DNA

The transcription factor Egr-1 specifically binds as a monomer to its 9-bp target DNA sequence, GCGTGGGCG, via three zinc fingers and plays important roles in the brain and cardiovascular systems. Using fluorescence-based competitive binding assays, we systematically analyzed the impacts of all poss...

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Vydáno v:Biochemistry
Hlavní autoři: Chattopadhyay, Abhijnan, Zandarashvili, Levani, Luu, Ross H., Iwahara, Junji
Médium: Artigo
Jazyk:Inglês
Vydáno: 2016
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5175577/
https://ncbi.nlm.nih.gov/pubmed/27933778
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.6b00757
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