High-level production of membrane proteins in E. coli BL21(DE3) by omitting the inducer IPTG

BACKGROUND: For membrane protein production, the Escherichia coli T7 RNA polymerase (T7 RNAP)-based protein production strain BL21(DE3) in combination with T7-promoter based expression vectors is widely used. Cells are routinely cultured in Lysogeny broth (LB medium) and expression of the chromosoma...

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Publicado en:Microb Cell Fact
Autores principales: Zhang, Zhe, Kuipers, Grietje, Niemiec, Łukasz, Baumgarten, Thomas, Slotboom, Dirk Jan, de Gier, Jan-Willem, Hjelm, Anna
Formato: Artigo
Lenguaje:Inglês
Publicado: BioMed Central 2015
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4574001/
https://ncbi.nlm.nih.gov/pubmed/26377812
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-015-0328-z
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