लोड हो रहा है...
High-level production of membrane proteins in E. coli BL21(DE3) by omitting the inducer IPTG
BACKGROUND: For membrane protein production, the Escherichia coli T7 RNA polymerase (T7 RNAP)-based protein production strain BL21(DE3) in combination with T7-promoter based expression vectors is widely used. Cells are routinely cultured in Lysogeny broth (LB medium) and expression of the chromosoma...
में बचाया:
| में प्रकाशित: | Microb Cell Fact |
|---|---|
| मुख्य लेखकों: | , , , , , , |
| स्वरूप: | Artigo |
| भाषा: | Inglês |
| प्रकाशित: |
BioMed Central
2015
|
| विषय: | |
| ऑनलाइन पहुंच: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4574001/ https://ncbi.nlm.nih.gov/pubmed/26377812 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-015-0328-z |
| टैग : |
टैग जोड़ें
कोई टैग नहीं, इस रिकॉर्ड को टैग करने वाले पहले व्यक्ति बनें!
|