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High-level production of membrane proteins in E. coli BL21(DE3) by omitting the inducer IPTG
BACKGROUND: For membrane protein production, the Escherichia coli T7 RNA polymerase (T7 RNAP)-based protein production strain BL21(DE3) in combination with T7-promoter based expression vectors is widely used. Cells are routinely cultured in Lysogeny broth (LB medium) and expression of the chromosoma...
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| Publicado no: | Microb Cell Fact |
|---|---|
| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4574001/ https://ncbi.nlm.nih.gov/pubmed/26377812 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-015-0328-z |
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