Tuning Escherichia coli for membrane protein overexpression

A simple generic method for optimizing membrane protein overexpression in Escherichia coli is still lacking. We have studied the physiological response of the widely used “Walker strains” C41(DE3) and C43(DE3), which are derived from BL21(DE3), to membrane protein overexpression. For unknown reasons...

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Detalhes bibliográficos
Main Authors: Wagner, Samuel, Klepsch, Mirjam M., Schlegel, Susan, Appel, Ansgar, Draheim, Roger, Tarry, Michael, Högbom, Martin, van Wijk, Klaas J., Slotboom, Dirk J., Persson, Jan O., de Gier, Jan-Willem
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2008
Assuntos:
Biological Sciences
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2567230/
https://ncbi.nlm.nih.gov/pubmed/18796603
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0804090105
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