Tuning Escherichia coli for membrane protein overexpression

A simple generic method for optimizing membrane protein overexpression in Escherichia coli is still lacking. We have studied the physiological response of the widely used “Walker strains” C41(DE3) and C43(DE3), which are derived from BL21(DE3), to membrane protein overexpression. For unknown reasons...

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Autors principals: Wagner, Samuel, Klepsch, Mirjam M., Schlegel, Susan, Appel, Ansgar, Draheim, Roger, Tarry, Michael, Högbom, Martin, van Wijk, Klaas J., Slotboom, Dirk J., Persson, Jan O., de Gier, Jan-Willem
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2008
Matèries:
Biological Sciences
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2567230/
https://ncbi.nlm.nih.gov/pubmed/18796603
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0804090105
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