Tuning Escherichia coli for membrane protein overexpression

A simple generic method for optimizing membrane protein overexpression in Escherichia coli is still lacking. We have studied the physiological response of the widely used “Walker strains” C41(DE3) and C43(DE3), which are derived from BL21(DE3), to membrane protein overexpression. For unknown reasons...

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Bibliografische gegevens
Hoofdauteurs: Wagner, Samuel, Klepsch, Mirjam M., Schlegel, Susan, Appel, Ansgar, Draheim, Roger, Tarry, Michael, Högbom, Martin, van Wijk, Klaas J., Slotboom, Dirk J., Persson, Jan O., de Gier, Jan-Willem
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: National Academy of Sciences 2008
Onderwerpen:
Biological Sciences
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2567230/
https://ncbi.nlm.nih.gov/pubmed/18796603
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0804090105
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