Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway

BACKGROUND: Heterologous expression systems based on promoters inducible with isopropyl-β-D-1-thiogalactopyranoside (IPTG), e.g., Escherichia coli BL21(DE3) and cognate LacI(Q)/P(lacUV5)-T7 vectors, are commonly used for production of recombinant proteins and metabolic pathways. The applicability of...

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Detalhes bibliográficos
Publicado no:Microb Cell Fact
Main Authors: Dvorak, Pavel, Chrast, Lukas, Nikel, Pablo I., Fedr, Radek, Soucek, Karel, Sedlackova, Miroslava, Chaloupkova, Radka, de Lorenzo, Víctor, Prokop, Zbynek, Damborsky, Jiri
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2015
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4687329/
https://ncbi.nlm.nih.gov/pubmed/26691337
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-015-0393-3
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