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High-throughput determination of RNA structure by proximity ligation

We present an unbiased method to globally resolve RNA structures through pairwise contact measurements between interacting regions. RNA Proximity Ligation (RPL) uses proximity ligation of native RNA followed by deep sequencing to yield chimeric reads with ligation junctions in the vicinity of struct...

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Détails bibliographiques
Publié dans:Nat Biotechnol
Auteurs principaux: Ramani, Vijay, Qiu, Ruolan, Shendure, Jay
Format: Artigo
Langue:Inglês
Publié: 2015
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4564351/
https://ncbi.nlm.nih.gov/pubmed/26237516
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.3289
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