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High-throughput determination of RNA structure by proximity ligation

We present an unbiased method to globally resolve RNA structures through pairwise contact measurements between interacting regions. RNA Proximity Ligation (RPL) uses proximity ligation of native RNA followed by deep sequencing to yield chimeric reads with ligation junctions in the vicinity of struct...

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Veröffentlicht in:Nat Biotechnol
Hauptverfasser: Ramani, Vijay, Qiu, Ruolan, Shendure, Jay
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2015
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4564351/
https://ncbi.nlm.nih.gov/pubmed/26237516
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.3289
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