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Use of double-stranded DNA mini-circles to characterize the covalent topoisomerase-DNA complex

The enzymatic DNA relaxation requires the DNA to be transiently nicked and rejoined, the covalent topoisomerase-DNA complex being a key intermediate of the nicking-joining reaction. Practically, this reaction is most often characterized by oligonucleotides. However, the incision-religation of an oli...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Millet, Armêl, Strauss, François, Delagoutte, Emmanuelle
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4547392/
https://ncbi.nlm.nih.gov/pubmed/26300432
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep13154
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