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Use of double-stranded DNA mini-circles to characterize the covalent topoisomerase-DNA complex
The enzymatic DNA relaxation requires the DNA to be transiently nicked and rejoined, the covalent topoisomerase-DNA complex being a key intermediate of the nicking-joining reaction. Practically, this reaction is most often characterized by oligonucleotides. However, the incision-religation of an oli...
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| Published in: | Sci Rep |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Nature Publishing Group
2015
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4547392/ https://ncbi.nlm.nih.gov/pubmed/26300432 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep13154 |
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