Hydride bridge in [NiFe]-hydrogenase observed by nuclear resonance vibrational spectroscopy

The metabolism of many anaerobes relies on [NiFe]-hydrogenases, whose characterization when bound to substrates has proven non-trivial. Presented here is direct evidence for a hydride bridge in the active site of the (57)Fe-labelled fully reduced Ni-R form of Desulfovibrio vulgaris Miyazaki F [NiFe]...

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Udgivet i:Nat Commun
Main Authors: Ogata, Hideaki, Krämer, Tobias, Wang, Hongxin, Schilter, David, Pelmenschikov, Vladimir, van Gastel, Maurice, Neese, Frank, Rauchfuss, Thomas B., Gee, Leland B., Scott, Aubrey D., Yoda, Yoshitaka, Tanaka, Yoshihito, Lubitz, Wolfgang, Cramer, Stephen P.
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 2015
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4531378/
https://ncbi.nlm.nih.gov/pubmed/26259066
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms8890
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