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Chemical genomic profiling via barcode sequencing to predict compound mode of action

Chemical genomics is an unbiased, whole-cell approach to characterizing novel compounds to determine mode of action and cellular target. Our version of this technique is built upon barcoded deletion mutants of Saccharomyces cerevisiae and has been adapted to a high-throughput methodology using next-...

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Bibliographische Detailangaben
Veröffentlicht in:Methods Mol Biol
Hauptverfasser: Piotrowski, Jeff S., Simpkins, Scott W., Li, Sheena C., Deshpande, Raamesh, McIlwain, Sean, Ong, Irene, Myers, Chad L., Boone, Charlie, Andersen, Raymond J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2015
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4497522/
https://ncbi.nlm.nih.gov/pubmed/25618354
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2269-7_23
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