Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli

To date, most genetic engineering approaches coupling the type II Streptococcus pyogenes clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system to lambda Red recombineering have involved minor single nucleotide mutations. Here we show that procedures for carrying out more comp...

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Vydáno v:Appl Environ Microbiol
Hlavní autoři: Pyne, Michael E., Moo-Young, Murray, Chung, Duane A., Chou, C. Perry
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2015
Témata:
Biotechnology
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4495200/
https://ncbi.nlm.nih.gov/pubmed/26002895
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01248-15
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