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Development of a CRISPR-Cas9 Tool Kit for Comprehensive Engineering of Bacillus subtilis

The establishment of a clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system for strain construction in Bacillus subtilis is essential for its progression toward industrial utility. Here we outline the development of a CRISPR-Cas9 tool kit for comprehensive genetic engineerin...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Main Authors: Westbrook, Adam W., Moo-Young, Murray, Chou, C. Perry
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4968543/
https://ncbi.nlm.nih.gov/pubmed/27260361
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01159-16
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