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Development of a CRISPR-Cas9 Tool Kit for Comprehensive Engineering of Bacillus subtilis
The establishment of a clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system for strain construction in Bacillus subtilis is essential for its progression toward industrial utility. Here we outline the development of a CRISPR-Cas9 tool kit for comprehensive genetic engineerin...
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| Pubblicato in: | Appl Environ Microbiol |
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| Autori principali: | , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
American Society for Microbiology
2016
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4968543/ https://ncbi.nlm.nih.gov/pubmed/27260361 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01159-16 |
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