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Fragment Exchange Plasmid Tools for CRISPR/Cas9-Mediated Gene Integration and Protease Production in Bacillus subtilis

Since its discovery as part of the bacterial adaptative immune system, CRISPR/Cas has emerged as the most promising tool for targeted genome editing over the past few years. Various tools for genome editing in Bacillus subtilis have recently been developed, expanding and simplifying its potential de...

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Détails bibliographiques
Publié dans:Appl Environ Microbiol
Auteurs principaux: García-Moyano, Antonio, Larsen, Øivind, Gaykawad, Sushil, Christakou, Eleni, Boccadoro, Catherine, Puntervoll, Pål, Bjerga, Gro Elin Kjæreng
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2020
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7755240/
https://ncbi.nlm.nih.gov/pubmed/33097498
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02090-20
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