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Fragment Exchange Plasmid Tools for CRISPR/Cas9-Mediated Gene Integration and Protease Production in Bacillus subtilis

Since its discovery as part of the bacterial adaptative immune system, CRISPR/Cas has emerged as the most promising tool for targeted genome editing over the past few years. Various tools for genome editing in Bacillus subtilis have recently been developed, expanding and simplifying its potential de...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Main Authors: García-Moyano, Antonio, Larsen, Øivind, Gaykawad, Sushil, Christakou, Eleni, Boccadoro, Catherine, Puntervoll, Pål, Bjerga, Gro Elin Kjæreng
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7755240/
https://ncbi.nlm.nih.gov/pubmed/33097498
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02090-20
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