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Intramolecular circularization increases efficiency of RNA sequencing and enables CLIP-Seq of nuclear RNA from human cells

RNA sequencing (RNA-Seq) is a powerful tool for analyzing the identity of cellular RNAs but is often limited by the amount of material available for analysis. In spite of extensive efforts employing existing protocols, we observed that it was not possible to obtain useful sequencing libraries from n...

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Vydáno v:Nucleic Acids Res
Hlavní autoři: Chu, Yongjun, Wang, Tao, Dodd, David, Xie, Yang, Janowski, Bethany A., Corey, David R.
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2015
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4477644/
https://ncbi.nlm.nih.gov/pubmed/25813040
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv213
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