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Intramolecular circularization increases efficiency of RNA sequencing and enables CLIP-Seq of nuclear RNA from human cells

RNA sequencing (RNA-Seq) is a powerful tool for analyzing the identity of cellular RNAs but is often limited by the amount of material available for analysis. In spite of extensive efforts employing existing protocols, we observed that it was not possible to obtain useful sequencing libraries from n...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Chu, Yongjun, Wang, Tao, Dodd, David, Xie, Yang, Janowski, Bethany A., Corey, David R.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4477644/
https://ncbi.nlm.nih.gov/pubmed/25813040
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv213
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