Duplex-specific nuclease efficiently removes rRNA for prokaryotic RNA-seq

Next-generation sequencing has great potential for application in bacterial transcriptomics. However, unlike eukaryotes, bacteria have no clear mechanism to select mRNAs over rRNAs; therefore, rRNA removal is a critical step in sequencing-based transcriptomics. Duplex-specific nuclease (DSN) is an e...

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Bibliografske podrobnosti
Main Authors: Yi, Hana, Cho, Yong-Joon, Won, Sungho, Lee, Jong-Eun, Jin Yu, Hyung, Kim, Sujin, Schroth, Gary P., Luo, Shujun, Chun, Jongsik
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2011
Teme:
Methods Online
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3203590/
https://ncbi.nlm.nih.gov/pubmed/21880599
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr617
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