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Duplex-specific nuclease efficiently removes rRNA for prokaryotic RNA-seq
Next-generation sequencing has great potential for application in bacterial transcriptomics. However, unlike eukaryotes, bacteria have no clear mechanism to select mRNAs over rRNAs; therefore, rRNA removal is a critical step in sequencing-based transcriptomics. Duplex-specific nuclease (DSN) is an e...
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| Autori principali: | , , , , , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
2011
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3203590/ https://ncbi.nlm.nih.gov/pubmed/21880599 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr617 |
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