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Analysis of Nuclear RNA Interference (RNAi) in Human Cells by Subcellular Fractionation and Argonaute Loading

RNA interference (RNAi) is well known for its ability to regulate gene expression in the cytoplasm of mammalian cells. In mammalian cell nuclei, however, the impact of RNAi has remained more controversial. A key technical hurdle has been a lack of optimized protocols for the isolation and analysis o...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Nat Protoc
Egile Nagusiak: Gagnon, Keith T., Li, Liande, Janowski, Bethany A., Corey, David R.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 2014
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC4251768/
https://ncbi.nlm.nih.gov/pubmed/25079428
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.135
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