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Analysis of Nuclear RNA Interference (RNAi) in Human Cells by Subcellular Fractionation and Argonaute Loading

RNA interference (RNAi) is well known for its ability to regulate gene expression in the cytoplasm of mammalian cells. In mammalian cell nuclei, however, the impact of RNAi has remained more controversial. A key technical hurdle has been a lack of optimized protocols for the isolation and analysis o...

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Détails bibliographiques
Publié dans:Nat Protoc
Auteurs principaux: Gagnon, Keith T., Li, Liande, Janowski, Bethany A., Corey, David R.
Format: Artigo
Langue:Inglês
Publié: 2014
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4251768/
https://ncbi.nlm.nih.gov/pubmed/25079428
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.135
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