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Sequencing small genomic targets with high efficiency and extreme accuracy

The detection of minority variants in mixed samples demands methods for enrichment and accurate sequencing of small genomic intervals. We describe an efficient approach based on sequential rounds of hybridization with biotinylated oligonucleotides, enabling more than one-million fold enrichment of g...

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Detalles Bibliográficos
Publicado en:Nat Methods
Main Authors: Schmitt, Michael W., Fox, Edward J., Prindle, Marc J., Reid-Bayliss, Kate S., True, Lawrence D., Radich, Jerald P., Loeb, Lawrence A.
Formato: Artigo
Idioma:Inglês
Publicado: 2015
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4414912/
https://ncbi.nlm.nih.gov/pubmed/25849638
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3351
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