Cleavage Specificity of Mycobacterium tuberculosis ClpP1P2 Protease and Identification of Novel Peptide Substrates and Boronate Inhibitors with Anti-bacterial Activity

The ClpP1P2 protease complex is essential for viability in Mycobacteria tuberculosis and is an attractive drug target. Using a fluorogenic tripeptide library (Ac-X(3)X(2)X(1)-aminomethylcoumarin) and by determining specificity constants (k(cat)/K(m)), we show that ClpP1P2 prefers Met ≫ Leu > Phe...

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Détails bibliographiques
Publié dans:J Biol Chem
Auteurs principaux: Akopian, Tatos, Kandror, Olga, Tsu, Christopher, Lai, Jack H., Wu, Wengen, Liu, Yuxin, Zhao, Peng, Park, Annie, Wolf, Lisa, Dick, Lawrence R., Rubin, Eric J., Bachovchin, William, Goldberg, Alfred L.
Format: Artigo
Langue:Inglês
Publié: American Society for Biochemistry and Molecular Biology 2015
Sujets:
Enzymology
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4409261/
https://ncbi.nlm.nih.gov/pubmed/25759383
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M114.625640
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