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The clonal composition of human CD4+CD25+Foxp3+ cells determined by a comprehensive DNA-based multiplex PCR for TCRB gene rearrangements

The characterization of the T-cell receptor (TCR) repertoire of CD4+ regulatory T cells (T(R)) have been limited due to the RNA degradation that results following permeabilization and fixation as routinely used for intracellular staining of Foxp3. In the present study the clonal composition of human...

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Publicat a:J Immunol Methods
Autors principals: Scheinberg, Phillip, Melenhorst, Jan J., Hill, Brenna J., Keyvanfar, Keyvan, Barrett, A. John, Price, David A., Douek, Daniel C.
Format: Artigo
Idioma:Inglês
Publicat: 2007
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4402232/
https://ncbi.nlm.nih.gov/pubmed/17316678
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2007.01.005
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