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In vivo Elimination of Parental Clones in General and Site-directed Mutagenesis
The Eco29k I restriction endonuclease is a Sac II isoschizomer that recognizes the sequence 5’-CCGCGG-3’ and is encoded, along with the Eco29k I methylase, in the Escherichia coli strain 29k. We have expressed the Eco29k I restriction-methylation system (RM2) in E. coli strain TG1 to produce the str...
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| Veröffentlicht in: | J Immunol Methods |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2014
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4394739/ https://ncbi.nlm.nih.gov/pubmed/25523926 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2014.12.008 |
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