In vivo Elimination of Parental Clones in General and Site-directed Mutagenesis

The Eco29k I restriction endonuclease is a Sac II isoschizomer that recognizes the sequence 5’-CCGCGG-3’ and is encoded, along with the Eco29k I methylase, in the Escherichia coli strain 29k. We have expressed the Eco29k I restriction-methylation system (RM2) in E. coli strain TG1 to produce the str...

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Détails bibliographiques
Publié dans:J Immunol Methods
Auteurs principaux: Holland, Erika G., Acca, Felicity E., Belanger, Kristina M., Bylo, Mary E., Kay, Brian K., Weiner, Michael P., Kiss, Margaret M.
Format: Artigo
Langue:Inglês
Publié: 2014
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Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4394739/
https://ncbi.nlm.nih.gov/pubmed/25523926
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2014.12.008
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