Identification of metabolically stable 5′-phosphate analogs that support single-stranded siRNA activity

The ss-siRNA activity in vivo requires a metabolically stable 5′-phosphate analog. In this report we used crystal structure of the 5′-phosphate binding pocket of Ago-2 bound with guide strand to design and synthesize ss-siRNAs containing various 5′-phosphate analogs. Our results indicate that the el...

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Veröffentlicht in:Nucleic Acids Res
Hauptverfasser: Prakash, Thazha P., Lima, Walt F., Murray, Heather M., Li, Wenyu, Kinberger, Garth A., Chappell, Alfred E., Gaus, Hans, Seth, Punit P., Bhat, Balkrishen, Crooke, Stanley T., Swayze, Eric E.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2015
Schlagworte:
Chemical Biology and Nucleic Acid Chemistry
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4381071/
https://ncbi.nlm.nih.gov/pubmed/25753666
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv162
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